专利号 CN119876354B 标题 Full premix type fluorescent PCR kit and stabilizer 申请人 申联生物医药(上海)股份有限公司 优先权日 2025-01-24 公开日 2026-01-27 法律状态 ACTIVE (Google Patents, 2026-01-27) 方向 A 来源 https://patents.google.com/patent/CN119876354B/zh 摘要/首段 The invention discloses a full premix fluorescent PCR kit and a stabilizer, wherein the stabilizer comprises histidine and Taq enzyme aptamer, the concentration of the histidine is 0.01M-0.05M, the concentration of the Taq enzyme aptamer is 1nM-20nM, and at l 补充(Google Patents 页面正文摘录) CN119876354B - 一种全预混式荧光pcr试剂盒及稳定剂 - Google Patents Info Links Classifications Landscapes Abstract Description | | | | | --- | --- | --- | | 通道 | -20℃保存7d | 37℃保存7d | | FAM | 32.87 | 32.30 | | FAM | 36.13 | 35.10 | | VIC | 31.99 | 31.37 | | VIC | 34.14 | 34.89 | | | | | --- | --- | | 反应液1 | 配方同实施例3 | | 反应液2 | 反应液1的基础上添加5nM的Taq酶适配体 | | 反应液3 | 用市售商品化PCR mix(Premix Ex Taq(Probe qPCR),Takara R390)进行配制 | | 反应液4 | 用支持全预混的商品化PCRmix配制(翌圣11827) | | 反应液5 | 将反应液2中的20mM组氨酸替换为20mM精氨酸 | Claims (2) Priority Applications (1) | Application Number | Priority Date | Filing Date | Title | | --- | --- | --- | --- | | CN202510117901.7A [CN119876354B (zh)](/patent/CN119876354B/zh) | 2025-01-24 | 2025-01-24 | 一种全预混式荧光pcr试剂盒及稳定剂 | Applications Claiming Priority (1) | Application Number | Priority Date | Filing Date | Title | | --- | --- | --- | --- | | CN202510117901.7A [CN119876354B (zh)](/patent/CN119876354B/zh) | 2025-01-24 | 2025-01-24 | 一种全预混式荧光pcr试剂盒及稳定剂 | Publications (2) | Publication Number | Publication Date | | --- | --- | | CN119876354A [CN119876354A (zh)](/patent/CN119876354A/zh) | 2025-04-25 | | CN119876354B true [CN119876354B (zh)](/patent/CN119876354B/zh) | 2026-01-27 | Family ID=95444822 Family Applications (1) | Application Number | Title | Priority Date | Filing Date | | --- | --- | --- | --- | | CN202510117901.7A Active [CN119876354B (zh)](/patent/CN119876354B/zh) | 2025-01-24 | 2025-01-24 | 一种全预混式荧光pcr试剂盒及稳定剂 | Country Status (1) | Country | Link | | --- | --- | | CN (1) | [CN119876354B (zh)](/patent/CN119876354B/zh) | Families Citing this family (1) \* Cited by examiner, † Cited by third party | Publication number | Priority date | Publication date | Assignee | Title | | --- | --- | --- | --- | --- | | [CN120577522B (zh)](/patent/CN120577522B/zh) \* | 2025-05