URL: https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/real-time-pcr-reagents/taqman-real-time-master-mixes/lyo-ready.html TITLE: TaqMan Lyo-Ready qPCR Master Mixes ERROR: None ![Our Instagram](/content/dam/LifeTech/global/home-page/2019/taqman-hr-meganav.jpg) ![Our Facebook](/content/dam/LifeTech/global/promotions/meganav/2017/01-Jan/popular2-175x115.gif) ![Blog Behind the Bench](/content/dam/LifeTech/global/home-page/images/oligos-main-popular.jpg) ![Customer Experience Center (CEC)](/content/dam/LifeTech/global/promotions/meganav/2017/01-Jan/popular4-175x115.jpg) Search Search # TaqMan Lyo-Ready qPCR Master Mixes ![lyo-showcase]( "lyo-showcase") Reliable and storage-temperature-agnostic molecular diagnostics qPCR assays need to utilize reagents that are easily lyophilized and provide consistent performance even with non-ideal samples. TaqMan Lyo-Ready qPCR master mixes fulfill these requirements, making them highly suited to lyophilized qPCR assays using your chosen primers and probes. TaqMan Lyo-Ready qPCR master mixes are designed for consistent performance even in demanding applications, including virus and bacteria detection. Easy to lyophilize and fast to re-hydrate, they ensure consistency before and after lyophilization even in multiplex reactions. [Download 1-Step Brochure](https://documents.thermofisher.com/TFS-Assets/GSD/brochures/taqman-lyo-ready-one-step-qpcr-mastermix-excipient-brochure.pdf)       [Request a quote](/br/en/home/global/forms/life-science/lyo-ready-quote.html) [Download qPCR Brochure](https://documents.thermofisher.com/TFS-Assets/GSD/brochures/taqman-2.5x-lyo-ready-qpcr-master-mix-with-excipient-brochure.pdf)        [Request a demo](/br/en/home/global/forms/life-science/lyo-ready-sample.html) [Download Lyo Ready Whitepaper](https://documents.thermofisher.com/TFS-Assets/GSD/Reference-Materials/qpcr-mdx-lyo-ready-master-mixes-whitepaper.pdf) TaqMan Lyo-Ready qPCR master mixes are available in several formulations. Customization is also available and can be requested by contacting your Thermo Fisher Scientific representative or emailing [custom.solutions@thermofisher.com](mailto:custom.solutions@thermofisher.com). ## Which TaqMan Lyo-Ready master mix is right for your application? | | | | | | | | | --- | --- | --- | --- | --- | --- | --- | | | [TaqMan 2.5X Lyo-Ready 1-Step Master Mix **with excipient**](/order/catalog/product/C14031B001) | [TaqMan 2.5X Lyo-Ready qPCR Master Mix **with excipient**](/order/catalog/product/C14081B001) | TaqMan 5X Lyo-Ready 1-Step Master Mix | TaqMan 5X Lyo-Ready 1-Step Master Mix **with ROX** | TaqMan 5X Lyo-Ready qPCR Master Mix | TaqMan 5X Lyo-Ready qPCR Master Mix **with ROX** | | [**Request a quote**](/br/en/home/global/forms/life-science/lyo-ready-quote.html) | | | | | | | | **Target** | RNA, DNA | DNA | RNA, DNA | RNA, DNA | DNA | DNA | | **Concentration** | 2.5X | 2.5X | 5X | 5X | 5X | 5X | | **Excipient** | **Yes**, pre-mixed | **Yes**, pre-mixed | None | None | None | None | | **Passive reference dye** | None | None | None | ROX | None | ROX | | **Reverse transcriptase** | Thermostable M-MLV | N/A | Thermostable M-MLV | Thermostable M-MLV | N/A | N/A | | **Pre-PCR benchtop stability** | 4 hr\* | 4 hr\* | <2 hr\* | <2 hr\* | <2 hr\* | <2 hr\* | | **Multiplexing targets** | 5 | 5 | 5 | 3 | 5 | 3 | | **Sample SKU** | C14031SMP (1.5 mL) | C14081SMP (1.5 mL) | C1403SMP (1.5 mL) | C1402SMP (1.5 mL) | C1408SMP (1.5 mL) | C1407SMP (1.5 mL) | | [**Request a sample**](/br/en/home/global/forms/life-science/lyo-ready-sample.html) | | | | | | | | **This product is best for you if you are** | * New to lyophilization * Would like to develop and manufacture a lyophilized qPCR assay * Need ready-to-use reagents and comprehensive guidance on the process | | * Experiencedin lyophilization * Have your own excipient and lyophilization program * Need a qPCR mix optimized for consistent and reliable performance before and after lyophilization | | | | [TaqMan 2.5X Lyo-Ready 1-Step Master Mix **with excipient**](/order/catalog/product/C14031B001) [TaqMan 2.5X Lyo-Ready qPCR Master Mix **with excipient**](/order/catalog/product/C14081B001) TaqMan 5X Lyo-Ready 1-Step Master Mix TaqMan 5X Lyo-Ready 1-Step Master Mix **with ROX** TaqMan 5X Lyo-Ready qPCR Master Mix TaqMan 5X Lyo-Ready qPCR Master Mix **with ROX** **Target** RNA, DNA DNA RNA, DNA RNA, DNA DNA DNA **Concentration** 2.5X 2.5X 5X 5X 5X 5X **Excipient** **Yes**, pre-mixed **Yes**, pre-mixed None None None None **Passive reference dye** None None None ROX None ROX **Reverse transcriptase** Thermostable M-MLV N/A Thermostable M-MLV Thermostable M-MLV N/A N/A **Pre-PCR benchtop stability** 4 hr\* 4 hr\* <2 hr\* <2 hr\* <2 hr\* <2 hr\* **Multiplexing targets** 5 5 5 3 5 3 **Sample SKU** C14031SMP (1.5 mL) C14081SMP (1.5 mL) C1403SMP (1.5 mL) C1402SMP (1.5 mL) C1408SMP (1.5 mL) C1407SMP (1.5 mL) **This product is best for you if you are** \*Recommend setting up plate on ice and running immediately. Stability is assay dependent. Greater stability has been observed for some assays. ### Technical Literatures  [Brochure: TaqMan 2.5X Lyo-Ready qPCR Master Mix With Excipient](https://documents.thermofisher.com/TFS-Assets/GSD/brochures/taqman-2.5x-lyo-ready-qpcr-master-mix-with-excipient-brochure.pdf)  [Brochure: TaqMan 2.5X Lyo-Ready 1-Step qPCR Master Mix With Excipient](https://documents.thermofisher.com/TFS-Assets/GSD/brochures/taqman-lyo-ready-one-step-qpcr-mastermix-excipient-brochure.pdf)  [Whitepaper: Switch easily from liquid to lyophilized format using TaqMan Lyo -ready master mix with excipient](https://documents.thermofisher.com/TFS-Assets/GSD/Reference-Materials/qpcr-mdx-lyo-ready-master-mixes-whitepaper.pdf) TaqMan Lyo-Ready qPCR master mixes are reagents used in further manufacturing (lyophilization) and labeled “Caution: For use as a raw material in further manufacturing applications”, manufactured in an ISO 13485 certified and FDA registered facility, and optimized for exceptional lot-to-lot consistency, inhibitor tolerance, and multiplexing. ![About Us](/store/global-header-footer-static/global/svg/external-link-bold.svg) ![Careers](/store/global-header-footer-static/global/svg/external-link-bold.svg) ![Investors](/store/global-header-footer-static/global/svg/external-link-bold.svg) ![News](/store/global-header-footer-static/global/svg/external-link-bold.svg) ![Social Responsibility](/store/global-header-footer-static/global/svg/external-link-bold.svg) ![Brasil flag icon](/store/global-header-footer-static/global/images/flags/br.gif)