URL: https://www.neb.com/en-us/products/l4001-lyoprime-luna-probe-one-step-rt-qpcr-mix-with-udg TITLE: LyoPrime Luna® Probe One-Step RT-qPCR Mix with UDG ERROR: None Skip to main content Enable accessibility for low vision Open the accessibility menu ![Image 1](https://www.neb.com/en-us/products/l4001-lyoprime-luna-probe-one-step-rt-qpcr-mix-with-udg) ![Image 2: Spinner: Black decorative](https://cdn.userway.org/widgetapp/images/spin_bl.svg) ![Image 3](https://cdn.userway.org/widgetapp/images/body_bl.svg) ![Image 4: Spinner: Black decorative](https://cdn.userway.org/widgetapp/images/spin_bl.svg) [![Image 5: NEB Logo with Country Flag](https://media.neb.com/asset/e358a57c-65fc-40a5-8153-0d644b8e2a24/New_NEB_AU_logo.svg)![Image 6: NEB Logo with Country Flag](https://media.neb.com/asset/ffe4197a-66a6-4976-bdcd-f0359e260e65/New_NEB_AU_logo_reversed.svg)](https://www.neb.com/) [](https://www.neb.com/en-us/products/l4001-lyoprime-luna-probe-one-step-rt-qpcr-mix-with-udg) [_![Image 7](https://www.neb.com/en-us/products/l4001-lyoprime-luna-probe-one-step-rt-qpcr-mix-with-udg)![Image 8](https://www.neb.com/en-us/products/l4001-lyoprime-luna-probe-one-step-rt-qpcr-mix-with-udg)_](https://www.neb.com/en-au/quick-order)[_![Image 9](https://www.neb.com/en-us/products/l4001-lyoprime-luna-probe-one-step-rt-qpcr-mix-with-udg)![Image 10](https://www.neb.com/en-us/products/l4001-lyoprime-luna-probe-one-step-rt-qpcr-mix-with-udg)_](https://www.neb.com/en-au/cart/view-cart)[3 * [Peptide Binder Discovery Learn how mRNA display can streamline peptide binder discovery workflows. 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This product contains the same versatile features and strong performance as the liquid version: Luna® Probe One-Step RT-qPCR 4X Mix with UDG ([NEB #M3019](https://www.neb.com/en-au/products/m3019-luna-probe-one-step-rt-qpcr-4x-mix-with-udg)). * Simple, rapid rehydration ([view vial protocol](https://www.neb.com/en-au/protocols/protocol-for-lyoprime-luna-one-step-rt-qpcr-mix-with-udg-neb-l4001), [view 96-well plate protocol](https://www.neb.com/en-au/protocols/protocol-for-lyoprime-luna-one-step-rt-qpcr-mix-with-udg-96-well-plate-neb-l4001p)) * Eliminate cold chain shipping requirements * Multiplex up to 5 targets to increase throughput * Increase [reaction specificity and robustness](https://www.neb.com/en-au/tools-and-resources/feature-articles/using-aptamers-to-control-enzyme-activities-hot-start-taq-and-beyond)with our unique pairing of Luna WarmStart® RT and Hot Start _Taq_ * Prevent carryover contamination with Thermolabile UDG and dUTP included in optimized mix * Maintain RNA integrity with Murine RNase Inhibitor included in optimized mix * Eliminate pipetting errors with non-fluorescent blue tracking dye * Use with a variety of instrument platforms, including those that require a high or low ROX reference signal ![Image 24](https://www.neb.com/en-au/-/media/nebus/campaign/lyoprime/lyoprimeluna_upperi_content.png?rev=30e34e2cb18e4185b0eee7baa6d1fbc9&hash=3C32A9F05157A0D0EED42F24A896FFE8)![Image 25](https://www.neb.com/en-au/-/media/nebus/page-images/products/new-products/l4001p_390x251.jpg?sc_lang=en&rev=1d0263761a214d5fafed24b6a5f7f2ec&hash=E233DB145D683A5F4669918ED4B799CE) Reagents Supplied Protocols ## Featured Video ![Image 26: LyoPrime Luna L4001P plate format overview](https://www.neb.com/en-au/-/media/nebus/page-images/video-thumbnails/lyoprimelunaprobeonestep413420.jpg?rev=c0853ec63cf548599ed9597a60419d50&sc_lang=en-au&la=en-AU&h=146&w=260&hash=A690B0DC711B98480A91A257D4A3CEE2) +[](https://www.neb.com/en-au/tools-and-resources/video-library/lyoprime-luna-l4001p-96-well-plate-format-overview?autoplay=1) #### [LyoPrime Luna® (NEB #L4001P) 96-Well Plate Format Overview](https://www.neb.com/en-au/tools-and-resources/video-library/lyoprime-luna-l4001p-96-well-plate-format-overview) [View additional videos](https://www.neb.com/en-us/products/l4001-lyoprime-luna-probe-one-step-rt-qpcr-mix-with-udg#pdp-video-list) | Catalog # | Cat # | Concentration | Conc | Size | List Price | List Price | Your Price | Quantity | | --- | --- | --- | --- | --- | --- | --- | --- | --- | | L4001S | Not Applicable | 120 reactions | $400.00 | $400.00 | [Sign In](https://www.neb.com/en-au/account/sign-in)[Sign In](https://www.neb.com/en-au/account/sign-in) | -+ | | L4001P | Not Applicable | 96 reactions | $579.20 | $579.20 | [Sign In](https://www.neb.com/en-au/account/sign-in)[Sign In](https://www.neb.com/en-au/account/sign-in) | -+ | | | Add to Cart | | Please enter a quantity for at least one size | * * * ![Image 27](https://www.neb.com/en-au/-/media/icons/pdp_customorder_icon.svg?rev=f490df082a354b2299f0d65b8fe3fd6e&sc_lang=en-au) Need a custom/large volume order? [Contact us](https://www.neb.com/en-au/forms/contact-the-customized-solutions-team?tfa_9146=L4001%20LyoPrime%20Luna%C2%AE%20Probe%20One-Step%20RT-qPCR%20Mix%20with%20UDG&tfa_4473=) [Product Information](https://www.neb.com/en-us/products/l4001-lyoprime-luna-probe-one-step-rt-qpcr-mix-with-udg#)[Protocols, Manuals & Usage](https://www.neb.com/en-us/products/l4001-lyoprime-luna-probe-one-step-rt-qpcr-mix-with-udg#)[FAQs & Troubleshooting](https://www.neb.com/en-us/products/l4001-lyoprime-luna-probe-one-step-rt-qpcr-mix-with-udg#)[Citations & Technical Literature](https://www.neb.com/en-us/products/l4001-lyoprime-luna-probe-one-step-rt-qpcr-mix-with-udg#)[Quality, Safety & Legal](https://www.neb.com/en-us/products/l4001-lyoprime-luna-probe-one-step-rt-qpcr-mix-with-udg#)[TOP](https://www.neb.com/en-us/products/l4001-lyoprime-luna-probe-one-step-rt-qpcr-mix-with-udg#) Go To [Product Information](https://www.neb.com/en-us/products/l4001-lyoprime-luna-probe-one-step-rt-qpcr-mix-with-udg#) [Product Information](https://www.neb.com/en-us/products/l4001-lyoprime-luna-probe-one-step-rt-qpcr-mix-with-udg#)[Protocols, Manuals & Usage](https://www.neb.com/en-us/products/l4001-lyoprime-luna-probe-one-step-rt-qpcr-mix-with-udg#)[FAQs & Troubleshooting](https://www.neb.com/en-us/products/l4001-lyoprime-luna-probe-one-step-rt-qpcr-mix-with-udg#)[Citations & Technical Literature](https://www.neb.com/en-us/products/l4001-lyoprime-luna-probe-one-step-rt-qpcr-mix-with-udg#)[Quality, Safety & Legal](https://www.neb.com/en-us/products/l4001-lyoprime-luna-probe-one-step-rt-qpcr-mix-with-udg#) [BACK TO TOP](https://www.neb.com/en-us/products/l4001-lyoprime-luna-probe-one-step-rt-qpcr-mix-with-udg#) * ## Product Information The LyoPrime Luna Probe One-Step RT-qPCR Mix with UDG is offered in a [lyophilized](https://www.neb.com/Error/404?item=web%3a%7bDB645DE4-3A39-49B4-AA67-72D1DB38354A%7d%40en-AU)format, allowing for ambient/room temperature shipping and storage prior to use. It is designed for real-time detection of target RNA sequences using hydrolysis probes. Performance in multiplexing applications has been optimized, with sensitive, linear detection of up to 5 targets across a range of inputs. The stable cake can be resuspended to different concentrations to accommodate a variety of sample input volumes. It is available in 2 x 60 reaction glass vials or a 96-well plate pack size (1 reaction per well). These products can be customized (e.g. with the addition of primers and probes) – please [contact us](https://www.neb.com/en-au/forms/contact-the-customized-solutions-team)for more information. **Figure 1: LyoPrime Luna offers a robust, versatile RT-qPCR option in a shelf-stable lyophilized format** ![Image 28](https://www.neb.com/en-au/-/media/nebus/page-images/products/luna/l4001_lyoprime_productcomp_formats_07235362.png?sc_lang=en&rev=fb5331f0c3f9487b889ae781731b920c&hash=4CDA0496A04D1F8EBEA3D8B5616E7954) _The LyoPrime Luna® RT-qPCR Mix (NEB #L4001) offers the same versatile features as the Luna RT-qPCR 4X Mix ([NEB #M3019](https://www.neb.com/en-au/products/m3019-luna-probe-one-step-rt-qpcr-4x-mix-with-udg)) in a lyophilized format that can be shipped and stored at room temperature. It is available in glass vials or a 96-well plate._ The mix contains the necessary components for one-step RT-qPCR, including Luna WarmStart RT, Hot Start _Taq_ DNA Polymerase, dNTPs, and Murine RNase Inhibitor in an optimized buffer. Combining Hot Start _Taq_ DNA Polymerase with a WarmStart-activated reverse transcriptase allows for dual control of enzyme activity via reversible, aptamer-based inhibition. This temperature-dependent activation helps prevent undesirable non-specific priming and extension prior to thermocycling, enabling room temperature reaction set-up after rehydration. The engineered Luna WarmStart RT also possesses higher thermostability than many other RTs, allowing an optimal reaction temperature of 55°C. Additionally, the mix is formulated with a [unique passive reference dye](https://www.neb.com/en-au/luna/simpler-choice)that is compatible across a variety of instrument platforms, including those that require a high or low ROX reference signal. The sensitivity of RT-qPCR makes it important to minimize DNA contamination wherever possible. The inclusion of dUTP and thermolabile UDG prevents carryover contamination, where a product of a previous amplification can serve as the substrate of a subsequent reaction. Unlike _E.coli_ UDG, the thermolabile UDG is completely inactivated at temperatures above 50°C, thereby having no effect on qPCR performance. [... middle omitted — see footer ...] ### [Luna®Probe One-Step RT-qPCR 4X Mix with UDG (No ROX)](https://www.neb.com/en-au/products/m3029-luna-probe-one-step-rt-qpcr-4x-mix-with-udg-no-rox) [![Image 46](https://www.neb.com/en-au/-/media/catalog/product-images/e3010.jpg?rev=e85e1b408e6a4588add9300873cecdaa)](https://www.neb.com/en-au/products/e3010-luna-script-rt-supermix-kit) ### [LunaScript® RT SuperMix Kit](https://www.neb.com/en-au/products/e3010-luna-script-rt-supermix-kit) [![Image 47](https://www.neb.com/en-au/-/media/catalog/product-images/e1555.jpg?rev=491d94705bef4897ae5fd063e04964d7)](https://www.neb.com/en-au/products/e1555-lunascript-multiplex-one-step-rt-pcr-kit) ### [LunaScript®Multiplex One-Step RT-PCR Kit](https://www.neb.com/en-au/products/e1555-lunascript-multiplex-one-step-rt-pcr-kit) Item 1 of 4 Submit Restocking Order - [x] ## Ineligible item added to cart Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. 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