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www.meridianlifescience.com Master Mixes for Molecular Ambient-Temperature Stable Assays DNA Polymerases and qPCR/RT-qPCR Master Mixes Formulated for Lyophilization or Oven/Air-Drying Molecular diagnostic tests are progressively moving towards lyophilized and air-dried formats. There are several advantages for this, including room temperature shipping and storage, extended shelf-life and increased flexibility in sample volume. In order to be compatible with drying however, enzyme preparations must be glycerol-free and include specialized excipients that preserve the mixture as it is exposed to various conditions including freezing, temperature ramps, vacuum and dehydration. An ideal lyophilization formulation for example, should stabilize an enzyme in a freeze-dried format and allow very fast rehydration and reactivation of the enzyme preparations, without impacting its performance post rehydration.
Plates, vials or strips are filled with solution Solution is frozen solid Frozen solution undergoes primary (Sublimation) and secondary (Desorption) drying processes under vacuum Dried product in cake or bead form Key Steps in Lyophilization Process Food Testing Water Testing Environmental Blood Banking Genetic Screening Human Diagnostics Vet Health DNA Barcoding APPLICATION KEY * (Wet mix can be dispensed into vials, plates or strips & subsequently air-dried under temperature-controlled conditions) HOW IT WORKS Air-Dryable Air-Dryable™ qPCR Mix •  Simple and easy to use glycerol-free mix containing Taq polymerase, reaction buffer, dNTPs, MgCl2, and air-dry excipients •  Ideal for multiplex assays on POC diagnostic platforms or automated high-throughput instruments •  Compatible with a range of air-drying protocols to produce an ambient temperature stable mix •  Reduces cost and complexity Air-Dryable™ RT-qPCR Mix •  Simple and easy to use •  Ideal for multiplex assays on POC diagnostic platforms or automated high-throughput instruments •  Compatible with a range of air-drying protocols to produce an ambient temperature stable mix •  Reduces cost and complexity PRODUCT HIGHLIGHTS Full enzyme activity following air-drying Activity of Air-Dryable qPCR Mix in both wet and air-dried formats were compared by singleplex qPCR assay on 10-fold dilution mouse cDNA template. The air-dried mix showed no loss of activity and sensitivity when compared to freshly prepared wet mix up to the assay limit of detection.
PRODUCT HIGHLIGHTS High-tolerance to PCR inhibitors in sputum samples Amplification profile of a mouse RNA target spiked into samples containing 10%, 5% or 0% artificial sputum in Universal Transport Media (UTM). The data illustrates that the performance of Air-Dryable 1-Step RT-qPCR Mix (MDX095) exhibits high tolerance towards inhibitors present in artificial sputum and UTM.
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VOLUME REACTIONS Air-Dryable™ qPCR Mix, 4x MDX082 5 mL 1,000 Rxn 50 mL 10,000 Rxn Applications: Applications: Wet | Air-dried 0% UTM+Sputum 5% UTM+Sputum 10% UTM+Sputum PRODUCT CAT NO.
VOLUME REACTIONS Air-Dryable™ 1-Step RT-qPCR Mix, 4x MDX095 5 mL 1,000 Rxn 50 mL 10,000 Rxn www.meridianlifescience.com Air-Dryable™ Direct DNA qPCR Blood •  Glycerol-free mix containing Taq polymerase, reaction buffer, dNTPs, MgCl2, and air-dry excipients •  Specifically designed to overcome PCR inhibitors found in whole blood, plasma and serum •  Ideal for developing direct (extraction-free) qPCR assays that are ambient-temperature stable PRODUCT HIGHLIGHTS PRODUCT CAT NO.
VOLUME REACTIONS Air-Dryable™ Direct DNA qPCR Blood, 4x MDX092 5 mL 1,000 Rxn 50 mL 10,000 Rxn Applications: High reaction efficiency on plasma, serum and whole blood samples containing anticoagulants Plasmid DNA contains the target S. aureus, P. falciparum and Epstein-Barr virus was spiked into 10% serum or 10% K2-EDTA plasma and amplified in a triplex reaction using air-dried MDX092 format (red) and kits from supplier R (orange) and supplier T (blue). The results illustrate higher end florescence and better sensitivity with MDX092 than with mixes from supplier R and T.
Staphylococcus aureus Serum Plasma Plasmodium falciparum Epstein-Barr Virus Air-Dryable Continued PRODUCT CAT NO.
VOLUME REACTIONS Lyo-Ready™ qPCR Mix MDX021 5 mL 500 Rxn 100 mL 10,000 Rxn Lyo-Ready™ qPCR Mix 2.6x MDX023 4 mL 500 Rxn 100 mL 12,500 Rxn CMV EBV Cycle Cycle Amplification Plot Amplification Plot Adenovirus DNA Extraction Control Cycle Cycle Amplification Plot Amplification Plot Lyo-Ready Lyo-Ready™ qPCR Mix •  Ready-to-use and glycerol-free qPCR master mix formulated with a specialized blend of excipients •  Ideal for multiplex assays on automated high-throughput instruments •  Compatible with a range of lyophilization protocols to produce freeze-dried beads or cakes PRODUCT HIGHLIGHTS Powerful multiplexing capacity Three viral sequences, Cytomegalovirus (CMV) Adenovirus, Epstein-Barr Virus (EBV) and a DNA Extraction Control were amplified with equal efficiency from synthetic DNA templates with Lyo-Ready qPCR Mix in a quadruplex qPCR probe assay.
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VOLUME REACTIONS Lyo-Ready™ 1-Step RT-qPCR Mix MDX024 10 mL 1,000 Rxn 100 mL 10,000 Rxn Lyo-Ready™ 1-Step RT-qPCR Virus Mix MDX062 10 mL 1,000 Rxn 100 mL 10,000 Rxn Lyo-Ready™ 1-Step RT-qPCR Mix •  Glycerol-free RT-qPCR master mix formulated with a specialized blend of excipients •   Suited for multiplex assays and low-copy number targets •  Virus mix (MDX062) is optimized for amplification of RNA or DNA viruses with high secondary structure from either extracted or intact virus samples Applications: Adenovirus Rotavirus A Cycle PRODUCT HIGHLIGHTS High sensitivity amplification from both DNA and RNA templates Rotavirus A (dsRNA) and Adenovirus (dsDNA) were amplified in a single multiplexed RT-qPCR assay using inactivated crude viral lysates and Lyo-Ready 1-Step RT-qPCR Mix. The result illustrates that both viruses were amplified with high sensitivity, demonstrating the ability of Lyo-Ready 1-Step RT-qPCR Mix to detect low-copy number RNA and DNA targets simultaneously from a single sample.
www.meridianlifescience.com PRODUCT CAT NO.
VOLUME REACTIONS Lyo-Ready™ LAMP Mix MDX097 5 mL 800 Rxn 50 mL 8,000 Rxn Lyo-Ready™ LAMP Mix •  Ideal for POC diagnostic platforms or automated high-throughput instruments •  Optimized for Loop-Mediated Isothermal DNA Amplification (LAMP) •  Concentrated 4x master mix •  Contains all the required excipients for subsequent lyophilization Applications: PRODUCT HIGHLIGHTS Better Sensitivity with Lower Sample Input The average time to results (TTR) for Lyo-Ready LAMP Mix (orange) and a mix from supplier O (tan) and supplier N (blue) were compared using a 10-fold serial dilution for the of BRCA1 gene (1,000, 100 and 10 copies). The relative concentrations of LAMP oligos were optimized to obtain earlier TTR compared to other experiments. The results demonstrate the increased sensitivity and greater reproducibility of the Lyo-Ready LAMP Mix, with earlier TTR values and a lower SD when compared to other suppliers. Reactions were incubated at 65°C for 60 min and TTR were measured at 1:10 of end fluorescence.
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VOLUME REACTIONS Aptamer Taq HS (glycerol free) (50 U/μL) MDX015 20 µL 50 mL 500 µL 500 mL DNA Polymerase Aptamer Taq HS (Glycerol Free) •  Highly suited to multiplex, high-throughput viral detection assays requiring high specificity •  No activation step - reduce an assay run time by up to 15 minutes •  Convenient room temperature reaction set-up •  High enzyme concentration (50 U/μL) compatible with lyophilization protocols PRODUCT HIGHLIGHTS Fast Hot-Start Comparison of qPCR performance of Aptamer Taq HS (glycerol free) vs an antibody hot-start Taq. PCR reactions were run with and without an initial 2 minute high temperature activation step for both polymerases and Ct values were compared. No difference was observed in the Ct values between the reactions for the Aptamer Taq HS (glycerol free), indicating that an activation step does not have an effect on polymerase performance. In contrast, the antibody hot-start Taq had a lower Ct value in the reaction with a 2 min high-temperature denaturation step indicating a improvement in performance over the reaction with no activation step. Overall, the data illustrates the immediate activation of the Aptamer Taq HS (glycerol free) allowing for faster hot-start and faster reaction protocols.
Applications: Condition MDX097 | Supplier O | Supplier N Average TTR MDX097 MDX097 MDX097 Supplier O Supplier O Supplier O Supplier N Supplier N Supplier N 0 5 10 1,000 copies 100 copies 10 copies 15 20 PRODUCT CAT NO.
VOLUME REACTIONS Lyo-compatible MMLV-RT MDX042 8 µL 1,000 Rxn 80 µL 10,000 Rxn Reverse Transcriptase Lyo-compatible MMLV-RT •  High-concentration MMLV-RT suitable for incorporation into lyophilized RT-PCR assays •  Demonstrates high efficiency in RT-qPCR assays and high sensitivity detection of low copy number RNA targets Applications: 04/21 ISO 13485 Certified Connect with us: Ordering information: USA 5171 Wilfong Road Memphis, Tennessee 38134 Fax: +1 901-333-8223 Toll Free: +1 800 327 6299 Email: info@meridianlifescience.com Orders: orders@meridianlifescience.com www.MeridianLifeScience.com To test a sample or to ask additional questions, E: info@meridianlifescience.com PRODUCT HIGHLIGHTS Comparison of freshly prepared and lyophilized glycerol-free Taq HS master mixes A 10-fold serial dilution of template DNA was used to set up two sets of qPCR assays. One set was lyophilized (blue) and the other left as a wet mix (black). Results illustrate that lyophilization of Glycerol-Free Taq HS does not have an effect on the quality of the qPCR, as both the assays demonstrate identical efficiency and sensitivity.
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VOLUME REACTIONS Glycerol-Free Taq HS 50 U/µL MDX011 20 µL 1,000 Units 500 µL 25,000 Units Lyo-Ready qPCR Buffer 2.5x MDX022 4 mL 1,000 Rxn 100 mL 12,500 Rxn Glycerol-Free Taq HS 50 U/µL •   Lyophilization-compatible DNA polymerase ideal for automated high-throughput testing •  Provided as separate ‘tubes’ of Taq, hot-start antibody, and enzyme dilution buffer •  Can be used with Lyo-Ready qPCR Buffer (Cat #MDX022) to develop lyophilized qPCR mixes Applications: