# 作者索取函 · 批量版（12 封 · 已更新） > 更新 2026-09-12｜**已剔除本轮手动下载到手的篇目**（PNAS×4 / NAR×2 / JBC 海藻糖 / Genome Res / Paik 2021 等 13 篇已入库）。 > 使用：复制 → 填通讯作者姓氏与邮箱（论文首页 Corresponding author / 或 ResearchGate 站内信）→ 发出 → 48 h 无回应跟进一轮。 > **邮箱不臆造**：本文件不预填任何邮箱，须从论文首页取得。 --- ## 1. [P0] The ice nucleation temperature determines the primary drying rate of lyophilization for samples frozen on a temperature-controlled shelf `10.1002/jps.1039` ｜ J Pharm Sci ｜ 2001 ｜ 出版社 Elsevier ｜ 支撑 D2 **Subject:** Request for reprint: The ice nucleation temperature determines the primary drying rate of lyophilization for samples frozen on a temperature-controlled shelf (J Pharm Sci, 2001) Dear Prof./Dr. [姓氏], I am a researcher at BIORI BIOTECH (Zhuhai, China), working on IVD raw materials (lyophilized isothermal amplification and qPCR premix). I am writing to kindly request a PDF copy of your paper "The ice nucleation temperature determines the primary drying rate of lyophilization for samples frozen on a temperature-controlled shelf" (J Pharm Sci, 2001; DOI: 10.1002/jps.1039). Our use is strictly internal technical evaluation — we will not redistribute or use it commercially. Thank you very much for your consideration. Best regards, [姓名] | BIORI BIOTECH, Marketing Center Email: [公司邮箱] **主题：** 索取论文全文：The ice nucleation temperature determines the primary drying rate of lyophilization for samples frozen on a temperature-controlled shelf（J Pharm Sci, 2001） 尊敬的 [姓氏] 老师： 您好。我在宝锐生物（BIORI BIOTECH，中国珠海）从事 IVD 原料技术工作（冻干等温扩增与 qPCR 全预混方向）。冒昧致信，希望索取您论文《The ice nucleation temperature determines the primary drying rate of lyophilization for samples frozen on a temperature-controlled shelf》（J Pharm Sci, 2001；DOI: 10.1002/jps.1039）的 PDF。 我们仅用于内部技术评估，不会转载或用于商业用途。 如蒙惠允，不胜感激。 此致 敬礼 [姓名] | 宝锐生物 营销中心 邮箱：[公司邮箱] --- ## 2. [P0] Hydrogen Bonding between Sugar and Protein Is Responsible for Inhibition of Dehydration-Induced Protein Unfolding `10.1006/abbi.1999.1175` ｜ Arch Biochem Biophys ｜ 1999 ｜ 出版社 Elsevier ｜ 支撑 D2 **Subject:** Request for reprint: Hydrogen Bonding between Sugar and Protein Is Responsible for Inhibition of Dehydration-Induced Protein Unfolding (Arch Biochem Biophys, 1999) Dear Prof./Dr. [姓氏], I am a researcher at BIORI BIOTECH (Zhuhai, China), working on IVD raw materials (lyophilized isothermal amplification and qPCR premix). I am writing to kindly request a PDF copy of your paper "Hydrogen Bonding between Sugar and Protein Is Responsible for Inhibition of Dehydration-Induced Protein Unfolding" (Arch Biochem Biophys, 1999; DOI: 10.1006/abbi.1999.1175). Our use is strictly internal technical evaluation — we will not redistribute or use it commercially. Thank you very much for your consideration. Best regards, [姓名] | BIORI BIOTECH, Marketing Center Email: [公司邮箱] **主题：** 索取论文全文：Hydrogen Bonding between Sugar and Protein Is Responsible for Inhibition of Dehydration-Induced Protein Unfolding（Arch Biochem Biophys, 1999） 尊敬的 [姓氏] 老师： 您好。我在宝锐生物（BIORI BIOTECH，中国珠海）从事 IVD 原料技术工作（冻干等温扩增与 qPCR 全预混方向）。冒昧致信，希望索取您论文《Hydrogen Bonding between Sugar and Protein Is Responsible for Inhibition of Dehydration-Induced Protein Unfolding》（Arch Biochem Biophys, 1999；DOI: 10.1006/abbi.1999.1175）的 PDF。 我们仅用于内部技术评估，不会转载或用于商业用途。 如蒙惠允，不胜感激。 此致 敬礼 [姓名] | 宝锐生物 营销中心 邮箱：[公司邮箱] --- ## 3. [P0] Lyophilization and development of solid protein pharmaceuticals `10.1016/S0378-5173(00)00423-3` ｜ Int J Pharm ｜ 2000 ｜ 出版社 Elsevier ｜ 支撑 D2 **Subject:** Request for reprint: Lyophilization and development of solid protein pharmaceuticals (Int J Pharm, 2000) Dear Prof./Dr. [姓氏], I am a researcher at BIORI BIOTECH (Zhuhai, China), working on IVD raw materials (lyophilized isothermal amplification and qPCR premix). I am writing to kindly request a PDF copy of your paper "Lyophilization and development of solid protein pharmaceuticals" (Int J Pharm, 2000; DOI: 10.1016/S0378-5173(00)00423-3). Our use is strictly internal technical evaluation — we will not redistribute or use it commercially. Thank you very much for your consideration. Best regards, [姓名] | BIORI BIOTECH, Marketing Center Email: [公司邮箱] **主题：** 索取论文全文：Lyophilization and development of solid protein pharmaceuticals（Int J Pharm, 2000） 尊敬的 [姓氏] 老师： 您好。我在宝锐生物（BIORI BIOTECH，中国珠海）从事 IVD 原料技术工作（冻干等温扩增与 qPCR 全预混方向）。冒昧致信，希望索取您论文《Lyophilization and development of solid protein pharmaceuticals》（Int J Pharm, 2000；DOI: 10.1016/S0378-5173(00)00423-3）的 PDF。 我们仅用于内部技术评估，不会转载或用于商业用途。 如蒙惠允，不胜感激。 此致 敬礼 [姓名] | 宝锐生物 营销中心 邮箱：[公司邮箱] --- ## 4. [P0] Effect of process conditions on recovery of protein activity after freezing and freeze-drying `10.1016/S0939-6411(98)00007-1` ｜ Eur J Pharm Biopharm ｜ 1998 ｜ 出版社 Elsevier ｜ 支撑 D2 **Subject:** Request for reprint: Effect of process conditions on recovery of protein activity after freezing and freeze-drying (Eur J Pharm Biopharm, 1998) Dear Prof./Dr. [姓氏], I am a researcher at BIORI BIOTECH (Zhuhai, China), working on IVD raw materials (lyophilized isothermal amplification and qPCR premix). I am writing to kindly request a PDF copy of your paper "Effect of process conditions on recovery of protein activity after freezing and freeze-drying" (Eur J Pharm Biopharm, 1998; DOI: 10.1016/S0939-6411(98)00007-1). Our use is strictly internal technical evaluation — we will not redistribute or use it commercially. Thank you very much for your consideration. Best regards, [姓名] | BIORI BIOTECH, Marketing Center Email: [公司邮箱] **主题：** 索取论文全文：Effect of process conditions on recovery of protein activity after freezing and freeze-drying（Eur J Pharm Biopharm, 1998） 尊敬的 [姓氏] 老师： 您好。我在宝锐生物（BIORI BIOTECH，中国珠海）从事 IVD 原料技术工作（冻干等温扩增与 qPCR 全预混方向）。冒昧致信，希望索取您论文《Effect of process conditions on recovery of protein activity after freezing and freeze-drying》（Eur J Pharm Biopharm, 1998；DOI: 10.1016/S0939-6411(98)00007-1）的 PDF。 我们仅用于内部技术评估，不会转载或用于商业用途。 如蒙惠允，不胜感激。 此致 敬礼 [姓名] | 宝锐生物 营销中心 邮箱：[公司邮箱] --- ## 5. [P0] Lyophilized Ready-to-Use Mix for the Real-Time Polymerase Chain Reaction Diagnosis `10.1021/acsabm.1c00131` ｜ ACS Appl Bio Mater ｜ 2021 ｜ 出版社 ACS ｜ 支撑 D2 **Subject:** Request for reprint: Lyophilized Ready-to-Use Mix for the Real-Time Polymerase Chain Reaction Diagnosis (ACS Appl Bio Mater, 2021) Dear Prof./Dr. [姓氏], I am a researcher at BIORI BIOTECH (Zhuhai, China), working on IVD raw materials (lyophilized isothermal amplification and qPCR premix). I am writing to kindly request a PDF copy of your paper "Lyophilized Ready-to-Use Mix for the Real-Time Polymerase Chain Reaction Diagnosis" (ACS Appl Bio Mater, 2021; DOI: 10.1021/acsabm.1c00131). Our use is strictly internal technical evaluation — we will not redistribute or use it commercially. Thank you very much for your consideration. Best regards, [姓名] | BIORI BIOTECH, Marketing Center Email: [公司邮箱] **主题：** 索取论文全文：Lyophilized Ready-to-Use Mix for the Real-Time Polymerase Chain Reaction Diagnosis（ACS Appl Bio Mater, 2021） 尊敬的 [姓氏] 老师： 您好。我在宝锐生物（BIORI BIOTECH，中国珠海）从事 IVD 原料技术工作（冻干等温扩增与 qPCR 全预混方向）。冒昧致信，希望索取您论文《Lyophilized Ready-to-Use Mix for the Real-Time Polymerase Chain Reaction Diagnosis》（ACS Appl Bio Mater, 2021；DOI: 10.1021/acsabm.1c00131）的 PDF。 我们仅用于内部技术评估，不会转载或用于商业用途。 如蒙惠允，不胜感激。 此致 敬礼 [姓名] | 宝锐生物 营销中心 邮箱：[公司邮箱] --- ## 6. [P0] Trehalose Crystallization During Freeze-Drying: Implications On Lyoprotection `10.1021/jz900338m` ｜ J Phys Chem Lett ｜ 2009 ｜ 出版社 ACS ｜ 支撑 D2 **Subject:** Request for reprint: Trehalose Crystallization During Freeze-Drying: Implications On Lyoprotection (J Phys Chem Lett, 2009) Dear Prof./Dr. [姓氏], I am a researcher at BIORI BIOTECH (Zhuhai, China), working on IVD raw materials (lyophilized isothermal amplification and qPCR premix). I am writing to kindly request a PDF copy of your paper "Trehalose Crystallization During Freeze-Drying: Implications On Lyoprotection" (J Phys Chem Lett, 2009; DOI: 10.1021/jz900338m). Our use is strictly internal technical evaluation — we will not redistribute or use it commercially. Thank you very much for your consideration. Best regards, [姓名] | BIORI BIOTECH, Marketing Center Email: [公司邮箱] **主题：** 索取论文全文：Trehalose Crystallization During Freeze-Drying: Implications On Lyoprotection（J Phys Chem Lett, 2009） 尊敬的 [姓氏] 老师： 您好。我在宝锐生物（BIORI BIOTECH，中国珠海）从事 IVD 原料技术工作（冻干等温扩增与 qPCR 全预混方向）。冒昧致信，希望索取您论文《Trehalose Crystallization During Freeze-Drying: Implications On Lyoprotection》（J Phys Chem Lett, 2009；DOI: 10.1021/jz900338m）的 PDF。 我们仅用于内部技术评估，不会转载或用于商业用途。 如蒙惠允，不胜感激。 此致 敬礼 [姓名] | 宝锐生物 营销中心 邮箱：[公司邮箱] --- ## 7. [P0] Protein Stability During Freezing: Separation of Stresses and Mechanisms of Protein Stabilization `10.1080/10837450701481157` ｜ Pharm Dev Technol ｜ 2007 ｜ 出版社 T&F ｜ 支撑 D2 **Subject:** Request for reprint: Protein Stability During Freezing: Separation of Stresses and Mechanisms of Protein Stabilization (Pharm Dev Technol, 2007) Dear Prof./Dr. [姓氏], I am a researcher at BIORI BIOTECH (Zhuhai, China), working on IVD raw materials (lyophilized isothermal amplification and qPCR premix). I am writing to kindly request a PDF copy of your paper "Protein Stability During Freezing: Separation of Stresses and Mechanisms of Protein Stabilization" (Pharm Dev Technol, 2007; DOI: 10.1080/10837450701481157). Our use is strictly internal technical evaluation — we will not redistribute or use it commercially. Thank you very much for your consideration. Best regards, [姓名] | BIORI BIOTECH, Marketing Center Email: [公司邮箱] **主题：** 索取论文全文：Protein Stability During Freezing: Separation of Stresses and Mechanisms of Protein Stabilization（Pharm Dev Technol, 2007） 尊敬的 [姓氏] 老师： 您好。我在宝锐生物（BIORI BIOTECH，中国珠海）从事 IVD 原料技术工作（冻干等温扩增与 qPCR 全预混方向）。冒昧致信，希望索取您论文《Protein Stability During Freezing: Separation of Stresses and Mechanisms of Protein Stabilization》（Pharm Dev Technol, 2007；DOI: 10.1080/10837450701481157）的 PDF。 我们仅用于内部技术评估，不会转载或用于商业用途。 如蒙惠允，不胜感激。 此致 敬礼 [姓名] | 宝锐生物 营销中心 邮箱：[公司邮箱] --- ## 8. [P1] Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity `10.1039/b902504a` ｜ Lab Chip ｜ 2009 ｜ 出版社 RSC ｜ 支撑 D4 **Subject:** Request for reprint: Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity (Lab Chip, 2009) Dear Prof./Dr. [姓氏], I am a researcher at BIORI BIOTECH (Zhuhai, China), working on IVD raw materials (lyophilized isothermal amplification and qPCR premix). I am writing to kindly request a PDF copy of your paper "Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity" (Lab Chip, 2009; DOI: 10.1039/b902504a). Our use is strictly internal technical evaluation — we will not redistribute or use it commercially. Thank you very much for your consideration. Best regards, [姓名] | BIORI BIOTECH, Marketing Center Email: [公司邮箱] **主题：** 索取论文全文：Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity（Lab Chip, 2009） 尊敬的 [姓氏] 老师： 您好。我在宝锐生物（BIORI BIOTECH，中国珠海）从事 IVD 原料技术工作（冻干等温扩增与 qPCR 全预混方向）。冒昧致信，希望索取您论文《Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity》（Lab Chip, 2009；DOI: 10.1039/b902504a）的 PDF。 我们仅用于内部技术评估，不会转载或用于商业用途。 如蒙惠允，不胜感激。 此致 敬礼 [姓名] | 宝锐生物 营销中心 邮箱：[公司邮箱] --- ## 9. [P1] Synthetic evolutionary origin of a proofreading reverse transcriptase `10.1126/science.aaf5409` ｜ Science ｜ 2016 ｜ 出版社 AAAS ｜ 支撑 D4 **Subject:** Request for reprint: Synthetic evolutionary origin of a proofreading reverse transcriptase (Science, 2016) Dear Prof./Dr. [姓氏], I am a researcher at BIORI BIOTECH (Zhuhai, China), working on IVD raw materials (lyophilized isothermal amplification and qPCR premix). I am writing to kindly request a PDF copy of your paper "Synthetic evolutionary origin of a proofreading reverse transcriptase" (Science, 2016; DOI: 10.1126/science.aaf5409). Our use is strictly internal technical evaluation — we will not redistribute or use it commercially. Thank you very much for your consideration. Best regards, [姓名] | BIORI BIOTECH, Marketing Center Email: [公司邮箱] **主题：** 索取论文全文：Synthetic evolutionary origin of a proofreading reverse transcriptase（Science, 2016） 尊敬的 [姓氏] 老师： 您好。我在宝锐生物（BIORI BIOTECH，中国珠海）从事 IVD 原料技术工作（冻干等温扩增与 qPCR 全预混方向）。冒昧致信，希望索取您论文《Synthetic evolutionary origin of a proofreading reverse transcriptase》（Science, 2016；DOI: 10.1126/science.aaf5409）的 PDF。 我们仅用于内部技术评估，不会转载或用于商业用途。 如蒙惠允，不胜感激。 此致 敬礼 [姓名] | 宝锐生物 营销中心 邮箱：[公司邮箱] --- ## 10. [P1] Forty years of directed evolution and its continuously evolving technology toolbox: A review `10.1002/bit.28009` ｜ Biotechnol Bioeng ｜ 2022 ｜ 出版社 Wiley ｜ 支撑 D4 **Subject:** Request for reprint: Forty years of directed evolution and its continuously evolving technology toolbox: A review (Biotechnol Bioeng, 2022) Dear Prof./Dr. [姓氏], I am a researcher at BIORI BIOTECH (Zhuhai, China), working on IVD raw materials (lyophilized isothermal amplification and qPCR premix). I am writing to kindly request a PDF copy of your paper "Forty years of directed evolution and its continuously evolving technology toolbox: A review" (Biotechnol Bioeng, 2022; DOI: 10.1002/bit.28009). Our use is strictly internal technical evaluation — we will not redistribute or use it commercially. Thank you very much for your consideration. Best regards, [姓名] | BIORI BIOTECH, Marketing Center Email: [公司邮箱] **主题：** 索取论文全文：Forty years of directed evolution and its continuously evolving technology toolbox: A review（Biotechnol Bioeng, 2022） 尊敬的 [姓氏] 老师： 您好。我在宝锐生物（BIORI BIOTECH，中国珠海）从事 IVD 原料技术工作（冻干等温扩增与 qPCR 全预混方向）。冒昧致信，希望索取您论文《Forty years of directed evolution and its continuously evolving technology toolbox: A review》（Biotechnol Bioeng, 2022；DOI: 10.1002/bit.28009）的 PDF。 我们仅用于内部技术评估，不会转载或用于商业用途。 如蒙惠允，不胜感激。 此致 敬礼 [姓名] | 宝锐生物 营销中心 邮箱：[公司邮箱] --- ## 11. [P1] Generation of Large Libraries of Random Mutants in Bacillus subtilis `10.2144/97232rr01` ｜ BioTechniques ｜ 1997 ｜ 出版社 未核实 ｜ 支撑 D4 **Subject:** Request for reprint: Generation of Large Libraries of Random Mutants in Bacillus subtilis (BioTechniques, 1997) Dear Prof./Dr. [姓氏], I am a researcher at BIORI BIOTECH (Zhuhai, China), working on IVD raw materials (lyophilized isothermal amplification and qPCR premix). I am writing to kindly request a PDF copy of your paper "Generation of Large Libraries of Random Mutants in Bacillus subtilis" (BioTechniques, 1997; DOI: 10.2144/97232rr01). Our use is strictly internal technical evaluation — we will not redistribute or use it commercially. Thank you very much for your consideration. Best regards, [姓名] | BIORI BIOTECH, Marketing Center Email: [公司邮箱] **主题：** 索取论文全文：Generation of Large Libraries of Random Mutants in Bacillus subtilis（BioTechniques, 1997） 尊敬的 [姓氏] 老师： 您好。我在宝锐生物（BIORI BIOTECH，中国珠海）从事 IVD 原料技术工作（冻干等温扩增与 qPCR 全预混方向）。冒昧致信，希望索取您论文《Generation of Large Libraries of Random Mutants in Bacillus subtilis》（BioTechniques, 1997；DOI: 10.2144/97232rr01）的 PDF。 我们仅用于内部技术评估，不会转载或用于商业用途。 如蒙惠允，不胜感激。 此致 敬礼 [姓名] | 宝锐生物 营销中心 邮箱：[公司邮箱] --- ## 12. [P1] MLDE 训练集设计（正式版；现有 bioRxiv 预印本，关系未确证） `10.1016/j.cels.2021.07.008` ｜ Cell Systems ｜ 2021 ｜ 出版社 Cell Press ｜ 支撑 D4 **Subject:** Request for reprint: MLDE 训练集设计（正式版；现有 bioRxiv 预印本，关系未确证） (Cell Systems, 2021) Dear Prof./Dr. [姓氏], I am a researcher at BIORI BIOTECH (Zhuhai, China), working on IVD raw materials (lyophilized isothermal amplification and qPCR premix). I am writing to kindly request a PDF copy of your paper "MLDE 训练集设计（正式版；现有 bioRxiv 预印本，关系未确证）" (Cell Systems, 2021; DOI: 10.1016/j.cels.2021.07.008). Our use is strictly internal technical evaluation — we will not redistribute or use it commercially. Thank you very much for your consideration. Best regards, [姓名] | BIORI BIOTECH, Marketing Center Email: [公司邮箱] **主题：** 索取论文全文：MLDE 训练集设计（正式版；现有 bioRxiv 预印本，关系未确证）（Cell Systems, 2021） 尊敬的 [姓氏] 老师： 您好。我在宝锐生物（BIORI BIOTECH，中国珠海）从事 IVD 原料技术工作（冻干等温扩增与 qPCR 全预混方向）。冒昧致信，希望索取您论文《MLDE 训练集设计（正式版；现有 bioRxiv 预印本，关系未确证）》（Cell Systems, 2021；DOI: 10.1016/j.cels.2021.07.008）的 PDF。 我们仅用于内部技术评估，不会转载或用于商业用途。 如蒙惠允，不胜感激。 此致 敬礼 [姓名] | 宝锐生物 营销中心 邮箱：[公司邮箱]