TOTAL_PAGES=11
=== PAGE 1 ===
2026桔园罗氏PPT摘要
超过50年的生物技术228:33
CUSTOMBIOTECH
Foche
Penzberg拥有丰富的知识和专名投术的积累
> 2.000 QC methods
6000 employees
from 57 countries
14.000 production
events/year
additional 17.000 bulk filling
events/year for Custom Biotech
>15.000products
选择信赖，让我们火您的5保驾护航
CUSTOMBIOTECH
Roche
保证产品供应和服务
生产可扩展（Procuction scalability）
我们的彭茨贝格（Penzberg）基地已累计生产超过 40,000 升核苷酸原料
已成功交付超过1吨的蛋白酶K（Proteinase K）酶蛋白
严苛技术指标下的一致性（Consistency within narrow specifications）
1000个连续批次的 dNTPs平均纯度高达99.9%
安全有保障的供应体系（Secured supply）
拥有完善的风险分析、灾难恢复计划以及变更控制程序
严苛的标準确保合规性 （Stringent standards to guarantee compliance）
每年成功通过超过25次客户质量审计
=== PAGE 2 ===
Taq酶热启动技术
17:34
CUSTOMBIOTECH
（Roche
Activation at 95°C
FastStart Taq
DNA Polymerase
Taq DNA
Polymerase
Hot Start technology
Hot start
time
Sensitivity &
specificity
stability
95°C/2 min
Chemical
+++
+++
modification
inactive
active
Antibody-based
++
++
inhibition
Active Tan DMA Polymerase + Seratved egte
Aptamer-based
十十+
inhioiton
Cl
0
AptaTaq Polymerase 16:59
CUSTOMBIOTECH
Roche
稳定性强
aSC No Aptamet
45CApLame
—6 months at -15 t0-252C
Vormalized Units （U /uL）
0t 好 吃 0t 吗
425CAptamer
- 6 months at +15 10 +25°C
B months at +210 +8°C
©37CNo Aptamer
X37C Aptamet
Hluorescence （483-533）
Time （weeks）
+45C Aptamner
-45'CNoApaame
io
15 20 25 30 35 60
Actlvity of fresh vs.drled enzyme stored al 37°C
G 20.
BaN
•
Plasmid DNA from Eurotium amstelodami （105
to 102 copies） using hydrolysis probes for
• fresh
• dried
qPCR tests.
• AptaTaq DNA Master is stable for 6 monthsat
+4°C and at +25°C
数粥孝源干罗氏诊断总郎
=== PAGE 3 ===
KAPA2G Fast 1kb/s
15:36
CUSTOMBIOTECH
Rocl
快速，耐受高GC
• Human genomic DNA （≤3.5 kb）and Lambda DNA
• Amplification of 5human gene fragments （5ng）
（≤5kb）
with different GC content （>65% in lanes 2 and 3）
compared to competitor Hotstart Taqs
• Reaction times are based on 35-cycle program
Total reaction time （min）
20z2四1路17582
Wid-type Taq
Hot Start
KAPA2G Fast
KAPA2G Fast
Competitor A
1hr14min
1hr05 min
Competitor Q
0.5 units （KAPA2G Fast HotStart, Competitor I）， 0.625 units
29min
KAPA2G
（Competitor A and Q）， extension （72°C） 1 seclcycle for
Fast, 60 sec/cycle for competitor enzym
數週来源于罗氏司
KAPA2G Robust
：15-30J3:54
CUSTOMBIOTECH
Roche
抑制剂耐受
Engineered to be morelfesistant to inhibitors
than wild-type Taq, enabling crude sample PCR，
• KAPA2G Robust HotStart Ready Mix compared to
with “quick-and-dirty" extractions.
wild-type Taq for performance on templates with
different CG-content
O 2 46 8 6 8 10 180 240 300 0 00t 0204 6008 G0063n0 ：
mM Nacl
41-80
61-70
81-∞0
Upper panel: KAPA2G Robust HotStart PCR Kit
Aplicon %GC
Lower panel: wilde-type Taq
数据来淇于罗氏诊斯总部
⑦
=== PAGE 4 ===
KAPA2G Fast Multiplex 3:12
CUSTOMBIOTECH
Roche
多重扩增，性能优异
KAPA
Corrpetlcr Q
Corpellr！
Routine MultiplexPCR
M2000g 502g 10ng 20g
KAPA
Corpelic Q
Copelir ！
Multiplex PCR of GC-rich DNA
80 minutes
89 minutes
数温来说于罗氏诊断总部
KAPASG HotStart DMA22i2 Fse
CUSTOMBIOTECH
Roche
快速扩增，1s延伸
99.916
89.916
Ampification Curves
79.916-
69.916
59.916
Fluorescence （465-510）
49.916
39.916
29.916
KAPA3G
19.916-
Comp.1
9.916
Comp.2
与其他Taq合酶相比，KAPA3G
-0.084
扩增效率更高，1s延伸时间，能
实现不同长度片段的稳定扩增。
5
10
15
20
Cycles
25
30
35
40
45
consistently high amplification curves. All polymerases were used according to manufacturer's
KAPA3G DNA Polymerase handles one-second extension and denaturation times with ease, producing
instructions （total assay run time: 23 minutes）.
甑报来淝干罗氏诊断总挪
=== PAGE 5 ===
KAPA3G Hot Start Master$38
CUSTOMBIOTECH
Roche
增表现良好
EGFR
Amplification Curves
中
：
ERBB2
Amplification Curves
（465-510）
Fluorescence
（533-580）
20
26
Cycles
28 30
32 34
36
38
40
-1.873
18
20
28 28 30 32 31
Cycles
36 38 t0
10.000
ACTB
Amplification Curves
6.000
8.000
— Singleplex
4,000
— Multiplex
2.000
0.000
26
28
30
Cycles
EGFR （FAM）， ERBB2 （Hex） and ACTB （Cy5）； Probe Assays; hgDNA 100ng - 0.1n
Singleplex vs. Multiplex results：
数据来源于罗氏诊漸总部
KAPA PROBE FORCE oPC. Nas3 3ix
CUSTOMBIOTECH
Roche
KAPA3G- a force in multiplexing
Isg20
B
0.35
0.40
F13a1
C
0.35
0.40
Camtal
D
40
Ube20
0.40
0.30
.35
Normalized Fluorescence
0.25
0.20
Normalized Fluorescence
0.25
0.10
0.15
0.20
0.15
Normalized Fluorescence
ggN吧g吼跳吼g
Normalized Fluorescence
gN吼goa
0.00
0.05
0.05
0.10
0.10
25
30
35
40
45
50
0.00
Cycle
25
30
35
Cycle
40
45
50
25
30
35
Cycle
40
45
50
0.00
25
30
35
Cycle
40 45 50
_ KAPA Probe Force
Competitor 1
Competitor 2
Competitor3
100 pg mouse gDNA （95°C for 30-，95*C for 3, 60'C fer 30）
数蛋来源于罗氏诊断总部
=== PAGE 6 ===
KAPA3G 广谱抑制剂耐县1:05
CUSTOMBIOTECH
〈Roche
复杂样本，精准检测
⑨
KAPA3G广谱抑制剂耐受
Purilied vs. inhibited sample ACq
秤丰料备方洼
Force
Probe
Competitor
Competitor
Competitor
Competitor
Competitor
•血红素 50），M
•直染 EDIA 02%
•血清 02%
100 pg human gDNA
29.62
28.91
29.08
29.53
29.78
Citrate （3.5 mM）
-0.04
2.64
-.18
0.98
0.20
290
•麦便 Q.05号
•鼻以子>05%
•疲渡05%
EDTA （2 mM）
024
-039
1.07
•黑色素>35ng/u
Blood inbibitors
Ferrtin （9 wg/10gL）
-0.33
0.50
0.48
10 mg
NA
NA
Hematin （100 JA）
0%9
075
NA
NA
Heparin ⑦50pg /10JL）
-023
1.14
0.59
圖3.用液体活检，姐织或有
DNA緊合鍊，耐受定义为Cp稿移＄3且災光250%別解总災光。
法所含有的多种抑制剂開出KAPASG
＜1 ACA
1-24Cq
2-3ACa
>3 4Cq
Detection failled Lowest concentr ation at which
Cq < 45 cycles delected or No Amplification （NA）.，
KAPA3G Hotstart Masl.0i9品剂对出
CUSTOMBIOTECH
Roche
以EDTA （3mM）为例.
KAPA3G
HotStart
Amplification Curves
Competitor 1 Amplification Curves
Fluorescence
（533-580）
Master
Fluorescence
（533-580）
18 20
24
26 28 30 32 $4 3638 t 62
Cycles
— WoEDTA
EDTA
Cycles
— Wo EDTA — EDTA
Competitor 2 Amplification Curves
Competitor 3 Amplification Curves
Fluorescence
（533-580）
Fluorescence
（533-580）
20
24
26
28 30 32 34
Cycles
— Wo EDTA
36
38 $ 42
EDTA
18 20
24 26
28 30 32 3436
Cycles
W/O EDTA
384424
— EDTA
ACTB （Hex）； hgDNA 100ng - 0.1ng
數识来潆于罗氏诊断总
◎
=== PAGE 7 ===
KAPA3G HotStart三重99:38
CUSTOMBIOTECH
冰刷剂表现
Roche
FAM
HEX
抑制剂
Cp-偏移
荧光
Gy5
s3
≥ 50%
Cp-偏移
荧光
SDS 0.01%
≥ 50%
Cp-偏移
≤3
荧光
≥ 50%
乙醇 34
EDTA 3mM
柠檬酸 3mM
尿素 180 mM
血色素 30pM
肝素 Q.1lU/ml
肌类0.25%
重亚硫酸盐 0.196
胆盐 0.07596
PCR抑制剂存在的情况下，KAPA3G也能实现可靠的多重扩增
数据米™于罗氏珍断总邰
KAPA3G HotStart直
08:48
CUSTOMBIOTECH
王三件）
Roche
◎
中
0.5%
0%-
1%
10%
5%
0% 05% 1% 6% 1% 15% 20%
10x 200m
15%
20%
30x 200m
Cp Values
0%
0.5%
1%
5%
10%
15%
20%
30.58 30.30
31.45
33.73
35.18
36.61
37.10
20% M
15%
KAPA3G DNA Polymerase excels in reactions containing up to 20% whole blood！
数据率跟于罗氏诊断总部
=== PAGE 8 ===
KAPA3G HotStart耐B⑨8:04
CUSTOMBIOTECH
（Roche，
助力肿瘤早筛
KAPA3G
Competilor A
Bisullite convert
Competitor B
Bisulfite
• 0.19
• H20 Control
02%
0.3%
= 0.496
• 0.75%6
0.590
數粥米源于罗氏诊断总擇
◎ 0
高浓度试剂提供更大#2935
CUSTOMBIOTECH
Roche
进一步提高检测灵敏度
Amplification Plot
中国IVD
1.2044④
75 pl sample volume
1.004+8-
25 ul sample vclume
6.00e+5
4,00e+5
2004+-
0.00+
20
25
Cydle
High concentration reagents enable more flexibility in assay design.
oo
=== PAGE 9 ===
反转录酶系列
06:07
CUSTOMBIOTECH
Roche
RT-PCR
One-enzymeRT-PCR system
Tth/205
205 Fast
Fast RT step in RT-PCR
EvoScript
单酶
True one-vial RT-PCR Master
Solely Mg？*-dependent RT-step
M-MLV RT
RT-PCR systems
The RT in two-enzyme
NxtScript RT
双酶
Improved stability up to 60°C lor RT-step
NxtScript 20RT
Highest stability: up to
70°C for RT-slep
37C
45°C
50C
BC
70C
M-MLV RT
NatSerty RT
NxtScript 2G/Transcriptor
/Z05 Fast/EvoScript
NxtScript with KAPA3G046647
CUSTOMBIOTECH
Roche
©
NxtScript -50°C
Total RNA （ng/rxn）
1000 ng
100ng
v--3.39+447
1ng
10ng
N-0:999
0.1ng
0.01ng
Low copy number detected at both
50°C and 65°C with NxtScript RT +
KAPA3G.
•NxtScript- 65°C
Cp for HPRT1 across range of Total RNA （ng/rxn）
1000 ng
100 ng
10ng
1ng
0.1ng
0.01ng
50°C
21.25
24.38
27.85
31.28
35.05
37.87
65°C
22.95
25.85
28.42
32.02
35.94
38.88
1-step multiplex rxn: target HPAT1
O grlng albgcg osCcfer 1B, 88Cher3,46ogcle 9sC ler5.6yc for1g
=== PAGE 10 ===
罗氏分子检测可冻干原件
04:20
CUSTOMBIOTECH
Roche
⑨◎
DNA Detection
RNA Detection
AptaTag DNA
KAPA3G DNA
HawkZ05 Fast
polymerases and
polymerase and
mastermixes
mastermix
mastermixes
polymerase and
NxtScript 2G
reverse transcriptase
Taq DNA polymerase
NxtScript
reverse transcriptase
Faster and capable of high
degrees of multiplexing
Robust and inhibitor
tolerant
No compromises needed
dNTPs, PCR Grade
03:26
CUSTOMBIOTECH
（Roche
纯度高8稳定性强
JCTP, ITTR.
n duTP: 35 days at 3940
•B。-J-心s
mg Prghetpt sail ONTP uchnon
纯度
PH
焦磷酸
99.9%
1000 lots of dNTPs
average purity of the last
稳定性：反复冻融50次
长效期：42个月
=== PAGE 11 ===
customBiotech 分于：07米6
CUSTOMBIOTECH
甲基化
中国I
FISH
NGS
dPCR
冻干
qPCR
Sanger测序
Nucleic acid
isolation & purification
Amplieaton Auxtlary reagents
Made for IVD