{
  "meta": {
    "title": "ATP 荧光检测四方差：易瑞、天隆、宝锐、克拉玛尔",
    "subtitle": "精密度、检测限、仪器与检测体系横向比较，以及宝锐切入天隆 ATP 需求的路径",
    "date": "2026-11-06",
    "version": "V01",
    "scope": "内部研究稿，含客户数据，禁止外发",
    "author": "宝锐生物 营销中心 销售管理"
  },
  "entities": {
    "baorui": {
      "name": "宝锐生物",
      "role": "荧光素酶原料供应商",
      "product": "E800-020714，1 mg/mL，甘油液体 / 冻干粉",
      "lod": "0.1 pmol ATP/管（3σ）",
      "linear": "0.1–200 pmol，R² ≥ 0.999",
      "precision": "未披露（无 CV、无空白 n 数、无加标回收）",
      "stability": "4℃ 90%、25℃ 77%、37℃ 74%（7 天活力保留）；客户实测 37℃ 73%",
      "signal": "同酶量 RLU 3,250 vs 对照 1,202，约 2.7 倍；单次实验，2T/点，未做显著性检验",
      "instrument": "无自有 ATP 仪器；数据来自 96 孔白板 / 手持式 ATP 检测仪",
      "price": "100 mg 1,600 元（16.0 元/mg）、500 mg 7,200 元（14.4 元/mg）、1 g 12,960 元（12.96 元/mg）",
      "cert": "ATP 系统认证未披露；酶原料 COA/批间数据待补",
      "data_quality": "2/5（有 LOD/线性/稳定性；缺系统 LOD、精密度、COA）"
    },
    "klamar": {
      "name": "克拉玛尔（Klamar）",
      "role": "生化试剂目录供应商",
      "product": "13081130021 萤火虫荧光素酶，冻干粉，SDS-PAGE ≥98%，15–30×10⁶ 光单位/mg 蛋白",
      "lod": "未披露",
      "linear": "未披露",
      "precision": "未披露",
      "stability": "无公开数据；易瑞体系 37℃ 7 天活力保留 40%",
      "signal": "无公开同酶量对比；易瑞项目中被宝锐 2.7 倍信号压过",
      "instrument": "无仪器",
      "price": "公开目录 1 mg 2,708 元、5 mg 5,423 元（约 1,085 元/mg）；内部定价表另记 100 mg 1,696.2 元（16.96 元/mg），与易瑞记录“约 100 元/mg”不一致",
      "cert": "未披露 COA、批间、应用验证",
      "data_quality": "2/5（有纯度/光单位/价格；缺 LOD/精密度/稳定性/COA）"
    },
    "tianlong": {
      "name": "天隆科技",
      "role": "ATP 仪器 + Quickswab 拭子系统厂家",
      "product": "Biolum / Biolum Plus / Biolum Pro；ATP Quickswab A010–A036",
      "lod": "营销标称 1×10⁻¹⁶ mol；官网正文 1×10⁻¹⁵ mol；AOAC 022401 系统 LOD 0.9 fmol",
      "linear": "Biolum 0–999,999 RLU；Biolum Pro R² ≥ 0.99",
      "precision": "AOAC 纯分析物 100 fmol 处 RSD 5.65%（开发者）/10.24%（独立实验室）；1 fmol 处 21.52%/75.18%；食品基质/微生物 RSD 约 14–52%",
      "stability": "拭子/试剂稳定性未公开；仪器工作温度 5–40℃",
      "signal": "AOAC 纯分析物 100 fmol 平均 RLU 237.4（开发者）/404.1（独立实验室）",
      "instrument": "Biolum：280 g、3.5 寸彩屏、10 秒、0–999,999 RLU、±5% 或 ±5 RLU、999 条、USB/蓝牙、10 h/500 h、倾角保护；Biolum Pro：420 g、5 寸触摸屏、10 秒、1×10⁻¹⁶ 标准品、R²≥0.99、1×10⁻¹³ mol CV<6%、10,000 条、云端",
      "price": "整机公开中标 18,000 元/台、19,700 元/台、2.9–3.0 万元/台；拭子/试剂价格未公开",
      "cert": "AOAC PTM 022401（2024-03-04 签发，证书标注有效期至 2024-12-31，续期需查最新列表）；NMPA、FDA、CE 宣称",
      "data_quality": "4/5（有系统 LOD/精密度/仪器/认证；缺酶供应商、采购价、拭子成本）"
    },
    "yirui": {
      "name": "易瑞生物",
      "role": "食品安全快检上市公司；天隆 Biolum 海外独家代理；ATP 试剂项目客户",
      "product": "ATP 荧光检测试剂（餐具/环境洁净度），客户自有 buffer；仪器型号未在内部记录中明确",
      "lod": "未披露；内部项目未做系统 LOD 对比",
      "linear": "未披露",
      "precision": "未披露；仅有加速稳定性对比",
      "stability": "宝锐 E800 37℃ 7 天 73%，Klamar 40%；易瑞要求 7 天下降≤15%、11 天≤25%",
      "signal": "宝锐同酶量信号约为 Klamar 3 倍（客户项目口径）",
      "instrument": "未公开自有 ATP 仪器；同时是天隆 Biolum 海外独家代理（除中国等少数国家）",
      "price": "易瑞首单 E800 100 mg / 100 mL，10,000 元，折 100 元/mg；年用量 3–5 g；1 mg 做 80–100 次测试",
      "cert": "2025 年营收 2.63 亿元、归母净利润 1,374.33 万元；70+ 国家；食品快检占 90.2%",
      "data_quality": "1/5（有应用与商业数据；缺仪器、LOD、精密度、buffer 细节）"
    }
  },
  "lod": [
    {"label": "天隆营销标称 1×10⁻¹⁶ mol", "fmol": 0.1, "pmol": 0.0001, "mol": "1e-16", "note": "参数表/产品页；仪器灵敏度口径"},
    {"label": "天隆 AOAC 022401 系统 LOD", "fmol": 0.9, "pmol": 0.0009, "mol": "9e-16", "note": "Biolum + ATP Test Swab，不锈钢表面"},
    {"label": "天隆官网正文 1×10⁻¹⁵ mol", "fmol": 1.0, "pmol": 0.001, "mol": "1e-15", "note": "Biolum 产品正文"},
    {"label": "宝锐 E800 0.1 pmol", "fmol": 100.0, "pmol": 0.1, "mol": "1e-13", "note": "96 孔板，3σ"}
  ],
  "precision": {
    "levels_fmol": [1, 5, 25, 100, 500, 1500],
    "developer_rsd": [21.52, 11.95, 5.78, 5.65, 9.62, 9.01],
    "independent_rsd": [75.18, 65.98, 16.50, 10.24, 12.76, 13.21],
    "note": "AOAC 022401 纯分析物，n=10；100 fmol = 0.1 pmol"
  },
  "stability": {
    "temps": ["4℃", "25℃", "37℃"],
    "baorui": [90, 77, 74],
    "klamar": [88, 55, 40],
    "target": 85,
    "note": "7 天活力保留率，0 天为 100%；宝锐客户实测 37℃ 73%；单次实验，2T/点，未做统计显著性检验"
  },
  "prices": [
    {"name": "宝锐 E800 100 mg", "price_per_mg": 16.0, "total": 1600, "note": "标准目录"},
    {"name": "宝锐 E800 500 mg", "price_per_mg": 14.4, "total": 7200, "note": "标准目录"},
    {"name": "宝锐 E800 1 g", "price_per_mg": 12.96, "total": 12960, "note": "标准目录"},
    {"name": "克拉玛尔 1 mg", "price_per_mg": 2708.0, "total": 2708, "note": "公开目录"},
    {"name": "克拉玛尔 5 mg", "price_per_mg": 1084.6, "total": 5423, "note": "公开目录"},
    {"name": "克拉玛尔 100 mg", "price_per_mg": 16.96, "total": 1696.2, "note": "内部定价表；与易瑞记录不一致"},
    {"name": "默克 L8507 100 mg", "price_per_mg": 47.26, "total": 4725.75, "note": "内部定价表对标"},
    {"name": "易瑞首单 100 mg", "price_per_mg": 100.0, "total": 10000, "note": "项目小批量价，不是目录价"}
  ],
  "case": {
    "timeline": [
      {"date": "2025–2026H1", "event": "累计赠样 701 份/mL（020702 696、020701 3、E800 2），处于竞品替换验证期"},
      {"date": "2026-10-19", "event": "技术交流：客户 buffer 中 E800 37℃ 7 天 73%，Klamar 40%；客户要求 7 天下降≤15%、11 天≤25%；年用量 3–5 g；1 mg 做 80–100 次测试；项目 30 万份/年，未来 100 万份/年；16 µL/test；200 nmol 标液"},
      {"date": "2026-10-23", "event": "客户拿回 1,500 个耗材管、配套仪器、标液，开始自测；已有初步意向"},
      {"date": "2026-11-04", "event": "ERP 首单：E800-020714 100 mL（100 mg），10,000 元，标准销售订单；折 100 元/mg"}
    ],
    "win": "在易瑞 ATP 试剂项目中，E800 以 37℃ 7 天 73–74% 对 Klamar 40% 的稳定性优势、约 2.7 倍信号和价格拉平，取得小批量商业采购。",
    "not_yet": "未完成全项准入：37℃ 7 天下降 26–27%，高于易瑞≤15%目标；没有同体系 LOD/精密度；没有易瑞仪器型号与 buffer 细节。",
    "price_note": "易瑞首单 100 元/mg 是项目小批量价；宝锐标准目录 100 mg 为 16 元/mg。两者相差 6.25 倍，不能把易瑞价当作天隆批量价。"
  },
  "sources": [
    {"id": 1, "name": "宝锐《荧光素酶产品资料 V02》", "detail": "2026-11-01，LOD、线性、稳定性、2.7 倍信号", "url": "对外物料/荧光素酶_产品资料_20261101_V02.html"},
    {"id": 2, "name": "宝锐《Luciferase A4 F版技术信息》", "detail": "2026-10-30，检测体系与规格", "url": "对外物料/Luciferase_A4_F版_技术信息_20261030_V01.html"},
    {"id": 3, "name": "宝锐《E800 荧光素酶标准产品定价方案》", "detail": "2026-09-22，价格、竞品对标、易瑞验证进展", "url": "产品与市场/调研报告/E800 荧光素酶标准产品定价方案-20260922-已填.xlsx"},
    {"id": 4, "name": "天隆 Biolum 产品页", "detail": "仪器参数、1×10⁻¹⁶/1×10⁻¹⁵ 两种写法、试剂开放化", "url": "https://www.medtl.com/products/instruments/other/18.html"},
    {"id": 5, "name": "天隆 Biolum Pro 产品页", "detail": "5 寸触摸屏、420 g、1×10⁻¹³ mol CV<6%、10,000 条", "url": "https://www.instrument.com.cn/show/C245203.html"},
    {"id": 6, "name": "AOAC RI Certificate 022401", "detail": "Biolum + ATP Test Swab，不锈钢表面，LOD 0.9 fmol；纯分析物与基质精密度", "url": "https://fobeventia.com/panel/assets/images/certificates/17151654422442AOAC%20-%20Biolum%20ATP%20Hygiene%20Monitoring%20System.pdf"},
    {"id": 7, "name": "天隆专利 CN203011835U", "detail": "ATP 荧光检测拭子结构；专利权人西安天隆；不涉及荧光素酶", "url": "https://patents.google.com/patent/CN203011835U/zh"},
    {"id": 8, "name": "天隆–易瑞战略合作新闻", "detail": "2025-09-04 签约；易瑞获 Biolum 海外独家代理（除中国等少数国家）；双品牌", "url": "https://www.medtl.com/focus-on-tianlong/company-news/322.html"},
    {"id": 9, "name": "易瑞生物 2025 年报/评级报告", "detail": "2025 营收 2.63 亿元、归母净利润 1,374.33 万元、70+ 国家、食品快检占 90.2%", "url": "https://www.cfi.net.cn/p20260427001583.html"},
    {"id": 10, "name": "克拉玛尔荧光素酶产品页", "detail": "13081130021；≥98% SDS-PAGE；15–30×10⁶ 光单位/mg；1 mg 2,708 元、5 mg 5,423 元", "url": "http://klamar-reagent.com/Productshow-13002.html"},
    {"id": 11, "name": "Promega ENLITEN ATP Assay TB267", "detail": "测量 10⁻¹¹–10⁻¹⁶ mol；CV≤10%；100 µL + 100 µL；10 秒积分", "url": "https://www.promega.com/-/media/files/resources/protocols/technical-bulletins/0/enliten-atp-assay-system-bioluminescence-detection-kit-for-atp-protocl.pdf"},
    {"id": 12, "name": "Merck 荧光素酶 ATP 灵敏度方法", "detail": "0.2 µg 蛋白、20 nM ATP 的酶活/灵敏度测试条件", "url": "https://www.sigmaaldrich.com/US/en/technical-documents/protocol/protein-biology/enzyme-activity-assays/enzymatic-assay-of-luciferase"},
    {"id": 13, "name": "Kotsiou et al. 2026", "detail": "ATP 生物发光医院表面卫生监测综述；检测器、表面、环境导致变异；不能区分微生物/非微生物 ATP", "url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13032431", "pmid": "41723553", "doi": "10.1186/s13756-026-01698-8"},
    {"id": 14, "name": "Bakke 2022", "detail": "ATP 检测实践与总腺苷酸检测进展；J Food Prot 85(7):1079–1095", "url": "https://pubmed.ncbi.nlm.nih.gov/35503956", "pmid": "35503956", "doi": "10.4315/JFP-21-384"},
    {"id": 15, "name": "Xu et al. 2019", "detail": "三种商业 ATP 生物发光系统比较；系统间差异", "url": "https://doi.org/10.1080/09603123.2018.1523983", "doi": "10.1080/09603123.2018.1523983"},
    {"id": 16, "name": "Sanna et al. 2018", "detail": "手术室 ATP 监测适用性与限制；BMC Infect Dis 18:583", "url": "https://pubmed.ncbi.nlm.nih.gov/30453892", "pmid": "30453892", "doi": "10.1186/s12879-018-3505-y"},
    {"id": 17, "name": "Koksharov et al. 2011", "detail": "萤火虫荧光素酶定向进化热稳定化；五点突变体 37℃ 半衰期 11.5 h，较野生型提高 44 倍", "url": "https://academic.oup.com/peds/article/24/11/835/1503578"},
    {"id": 18, "name": "Baggett et al. 2004", "detail": "荧光素酶热不稳定；体外 37℃ 半衰期约 3 min", "url": "https://pubmed.ncbi.nlm.nih.gov/15802049", "pmid": "15802049"},
    {"id": 19, "name": "MarketsandMarkets 中国快检市场", "detail": "2025 年 13.648 亿美元→2030 年 23.881 亿美元，CAGR 11.8%；宽口径，不是 ATP 专项", "url": "https://www.marketsandmarkets.com/Market-Reports/geography/rapid-food-safety-testing-market/china"},
    {"id": 20, "name": "Growth Market Reports ATP 卫生监测", "detail": "全球食品工厂 ATP 卫生监测 2025 年 5.15 亿美元→2034 年 11.02 亿美元，CAGR 8.8%；全球口径", "url": "https://growthmarketreports.com/report/atp-hygiene-monitoring-for-food-plants-market"},
    {"id": 21, "name": "天隆 Biolum 中标数据", "detail": "4 台，单价 19,700 元；另见 18,000 元/台、2.9–3.0 万元/台公开记录", "url": "https://www.antpedia.com/instrument/bidding/584/1376832.html"},
    {"id": 22, "name": "易瑞内部活动与订单", "detail": "2026-10-19 技术交流、2026-10-23 客户自测、2026-11-04 ERP 首单；客户口径", "url": "数据/daily_activities_mcp.json；数据/orders_detail_2026.json"}
  ]
}